This test method is a procedure that utilizes immunoaffinity columns (IACs) designed for the cleanup and concentration of multiple mycotoxins (Deoxynivalenol (DON), aflatoxins (B1, B2, G1, G2), ochratoxin, zearalenone, fumonisins (B1, B2, B3), T-2, and HT-2) from oil samples prior to analysis by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).