This test method is a procedure used to determine the amount of acetone-insoluble matter in vegetable lecithins. This test measures the percentage of material in lecithin that does not dissolve in acetone, primarily consisting of phospholipids, glycolipids, and other high molecular weight, non-oil components. In this method, a weighed sample of lecithin is mixed with acetone under controlled conditions. The insoluble matter is separated by centrifugation, dried, and weighed. The result is expressed as a percentage of the original sample.

The combustion method for acid detergent insoluble protein (ADIP) is used to measure the amount of indigestible protein bound within the fiber portion of animal feed, particularly relevant for assessing heat-damaged or low-quality proteins. In this method, the feed sample is treated with an acid detergent solution to isolate the acid detergent fiber (ADF), which includes cellulose, lignin, and associated protein. A portion of the ADF residue is analyzed using combustion to determine its nitrogen content, and the total nitrogen content is multiplied by a standard conversion factor to determine crude protein.

The Kjeldahl method for acid detergent insoluble protein (ADIP) is used to measure the amount of indigestible protein bound within the fiber portion of animal feed, particularly relevant for assessing heat-damaged or low-quality proteins. In this method, the feed sample is treated with an acid detergent solution to isolate the acid detergent fiber (ADF), which includes cellulose, lignin, and associated protein. A portion of the dried ADF residue is subjected to the Kjeldahl digestion process, and the measured total nitrogen content is multiplied by a standard conversion factor to determine crude protein.

This test method is a procedure used to determine Acid Detergent Lignin (ADL) in feedstuffs, forages, and fibrous materials. This method measures the lignin content—a complex, indigestible component of plant cell walls—after removing other fibrous materials. Lignin is important because it negatively impacts digestibility and nutrient availability in ruminant diets. The sample is treated with an acid detergent solution using the ANKOM Fiber Analyzer to isolate ADF (cellulose, lignin, and ash). The ADF residue in ANKOM filter bags is then treated with 72% sulfuric acid, which solubilizes the cellulose, leaving lignin and ash behind. Bags are rinsed, dried, and weighed to determine the combined lignin + ash content. To isolate lignin, samples may be ashed in a muffle furnace at 550°C and reweighed. The difference gives the lignin content alone.

This test method is a modified version of the standard ether extraction method for determining crude fat in animal feed and feed ingredients. This modification is used when samples contain bound fats (e.g., in high-fiber or heat-processed feeds) that are not fully extractable by ether alone. The sample is first treated with hydrochloric acid (HCl) and heated to break down protein-fat and carbohydrate-fat complexes, releasing bound lipids. The hydrolyzed mixture is extracted with ether solvents, and the solvent-extracted fats are collected. The solvents are evaporated, and the fat residue is dried and weighed.

This test method is a procedure used to determine the acid value (AV) of fats and oils. The acid value measures the amount of free fatty acids (FFA) present, which indicates the degree of hydrolytic rancidity or degradation in the fat or oil. The acid value is an important quality parameter because high FFA levels can negatively affect flavor, stability, and shelf life of edible fats and oils. A weighed amount of the fat or oil is dissolved in a neutral solvent mixture, then titrated with a standard solution of potassium hydroxide (KOH) using a phenolphthalein indicator until a faint pink endpoint is reached. The acid value is calculated as the number of milligrams of KOH required to neutralize the free fatty acids in 1 gram of sample.

This test method is a procedure used to determine Acid Detergent Fiber (ADF) content in forages, animal feeds, and fibrous materials. ADF represents the portion of plant material composed mainly of cellulose, lignin, and silica, which are less digestible components affecting the nutritional quality of the feed. The feed or forage sample is dried, ground, and weighed. Then the sample is boiled in an acid detergent solution, which removes hemicellulose and soluble components, leaving behind the ADF residue. The insoluble residue is filtered, dried, and weighed. The residue is ashed in a furnace to remove mineral content; the weight loss upon ashing is subtracted to correct the fiber weight.

This test method is based on Standard Methods for the Examination of Water and Wastewater and is used to determine alkalinity in water samples. Alkalinity measures the water’s capacity to neutralize acids. A measured volume of water sample is placed in a beaker. The sample is titrated with a standardized sulfuric or hydrochloric acid solution to a specific pH. Indicators (like methyl orange or phenolphthalein) or a pH meter are used to detect endpoints. Alkalinity is calculated and typically expressed as mg/L of CaCO₃.

This test method is a Performance Tested Method (PTM) validated by AOAC for the quantitative detection of egg proteins in food products and environmental samples using the Neogen Veratox® for Egg Allergen test kit. This method employs a sandwich ELISA (Enzyme-Linked Immunosorbent Assay) to detect trace amounts of egg white and yolk proteins, which are common food allergens.

This test method is a quantitative ELISA (Enzyme-Linked Immunosorbent Assay) designed to detect and measure gliadin, the alcohol-soluble protein component of gluten found in wheat, barley, and rye. This method is widely used to help food producers ensure compliance with gluten-free labeling regulations and to prevent gluten cross-contact in manufacturing environments.

This test method is a quantitative ELISA (Enzyme-Linked Immunosorbent Assay) designed to detect and measure peanut protein residues in foods, ingredients, environmental swabs, and clean-in-place (CIP) rinse waters. It is widely used in food manufacturing to support allergen control programs, sanitation verification, and labeling compliance.

This test method is a quantitative ELISA (Enzyme-Linked Immunosorbent Assay) designed to detect and measure soy protein residues in food products, ingredients, environmental swabs, and CIP (clean-in-place) rinse water. It is commonly used in food manufacturing and processing facilities to verify allergen control procedures, prevent cross-contact, and support regulatory compliance for soy allergen labeling.

This test method is a quantitative ELISA (Enzyme-Linked Immunosorbent Assay) designed to detect and measure total milk proteins (including casein, beta-lactoglobulin, and other whey proteins) in food products, ingredients, environmental swabs, and CIP (clean-in-place) rinse waters. It is used by food manufacturers to prevent milk allergen cross-contact, verify cleaning procedures, and comply with allergen labeling regulations.

This test method is a widely used technique to accurately quantify these sulfur-containing amino acids in food, feed, and biological samples. Because cysteine and methionine are sensitive to oxidation and do not naturally absorb UV light strongly, they require chemical modification (derivatization) prior to chromatographic analysis. Samples are first oxidized to stabilize these amino acids, then hydrolyzed to release them from proteins. The free amino acids are then derivatized with a reagent (e.g., AccQ•Tag™) to make them detectable by UV. The derivatized sample is separated by reversed-phase UPLC and quantified using the detector, allowing accurate measurement of cysteine and methionine in complex samples.

This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino acids are separated on a reversed-phase UPLC column and detected using UV detectors. Quantification is achieved by comparing to calibration standards. This test method quantifies alanine, arginine, aspartic acid, glutamic acid, glycine, histadine, hydroxyproline, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine.

This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino acids are separated on a reversed-phase UPLC column and detected using UV or fluorescence detectors. Quantification is achieved by comparing to calibration standards.

This test method involves chemically modifying tryptophan to form stable, UV- or fluorescence-detectable derivatives before chromatographic separation. Because tryptophan is sensitive to acid hydrolysis, alkaline hydrolysis is commonly used to release tryptophan without degradation. The derivatized tryptophan is then separated on a reversed-phase UPLC column and detected using UV or fluorescence detectors. This method allows accurate and sensitive quantification of tryptophan in a variety of sample types.

This test method is a classic analytical technique used to determine ammonia nitrogen (NH₃-N) in water samples. It involves alkalizing the sample to convert ammonium ions to ammonia gas, which is then distilled, captured in an acid solution, and quantified by titration. This method measures the concentration of ammonia as nitrogen and is widely used for regulatory and environmental water testing.

This test method is a colorimetric procedure used to determine ammonia nitrogen (NH₃-N) in water and wastewater samples. The method uses the automated phenate reaction, in which ammonia reacts with phenol and hypochlorite under alkaline conditions to form a blue-colored indophenol complex. The intensity of this color is measured spectrophotometrically and is directly proportional to the concentration of ammonia nitrogen in the sample.

This test method is a colorimetric procedure used to determine ammonia nitrogen (NH₃-N) in water and wastewater samples, with a pre-analysis distillation of the test sample. The method uses the automated phenate reaction, in which ammonia reacts with phenol and hypochlorite under alkaline conditions to form a blue-colored indophenol complex. The intensity of this color is measured spectrophotometrically and is directly proportional to the concentration of ammonia nitrogen in the sample.

This test method is a classic analytical technique used to determine ammonia nitrogen (NH₃-N) in feed samples. It involves alkalizing the sample to convert ammonium ions to ammonia gas, which is then distilled, captured in an acid solution, and quantified by titration. This method measures the concentration of ammonia as nitrogen and is widely used for regulatory and environmental water testing.