This test method is a simple, packed, volumetric technique used to measure the mass per unit volume of a granular or powdered material, including any voids between particles. This value is important for evaluating flow properties, packaging efficiency, and handling characteristics of materials like grains, powders, or animal feed.
This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino… Continue reading Amino Acids [Main] Pre-col AQC Der
This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino… Continue reading Amino Acids [Taurine] Pre-col AQC Der
This test method involves chemically modifying tryptophan to form stable, UV- or fluorescence-detectable derivatives before chromatographic separation. Because tryptophan is sensitive to acid hydrolysis, alkaline hydrolysis is commonly used to release tryptophan without degradation. The derivatized tryptophan is then separated on a reversed-phase UPLC column and detected using UV or fluorescence detectors. This method allows… Continue reading Amino Acids [Tryptophan] Pre-col AQC Der
The combustion method for acid detergent insoluble protein (ADIP) is used to measure the amount of indigestible protein bound within the fiber portion of animal feed, particularly relevant for assessing heat-damaged or low-quality proteins. In this method, the feed sample is treated with an acid detergent solution to isolate the acid detergent fiber (ADF), which… Continue reading Acid Detergent Insoluble Protein – Combustion
The Kjeldahl method for acid detergent insoluble protein (ADIP) is used to measure the amount of indigestible protein bound within the fiber portion of animal feed, particularly relevant for assessing heat-damaged or low-quality proteins. In this method, the feed sample is treated with an acid detergent solution to isolate the acid detergent fiber (ADF), which… Continue reading Acid Detergent Insoluble Protein – Kjeltec
This test method is a procedure used to determine Acid Detergent Lignin (ADL) in feedstuffs, forages, and fibrous materials. This method measures the lignin content—a complex, indigestible component of plant cell walls—after removing other fibrous materials. Lignin is important because it negatively impacts digestibility and nutrient availability in ruminant diets. The sample is treated with… Continue reading Acid Detergent Lignin [ADL]
This test method is a modified version of the standard ether extraction method for determining crude fat in animal feed and feed ingredients. This modification is used when samples contain bound fats (e.g., in high-fiber or heat-processed feeds) that are not fully extractable by ether alone. The sample is first treated with hydrochloric acid (HCl)… Continue reading Acid Hydrolysis Fat
This test method is a procedure used to determine Acid Detergent Fiber (ADF) content in forages, animal feeds, and fibrous materials. ADF represents the portion of plant material composed mainly of cellulose, lignin, and silica, which are less digestible components affecting the nutritional quality of the feed. The feed or forage sample is dried, ground,… Continue reading ADF
This test method is a widely used technique to accurately quantify these sulfur-containing amino acids in food, feed, and biological samples. Because cysteine and methionine are sensitive to oxidation and do not naturally absorb UV light strongly, they require chemical modification (derivatization) prior to chromatographic analysis. Samples are first oxidized to stabilize these amino acids,… Continue reading Amino Acids [Cysteine/Methionine] Pre-col AQC Der