Free Amino Nitrogen [FAN]

This test method is a classical ninhydrin colorimetric method used to determine free amino nitrogen (FAN) in malt, wort, and beer. FAN is an essential nutrient for yeast during fermentation, and its measurement is critical for assessing the fermentability and nutritional quality of brewing materials.

Fusels

This test method uses static headspace gas chromatography with flame ionization detection (GC–FID) to measure fusel alcohols—higher-chain alcohols produced during fermentation. The measurement of fusel alcohols is important for quality control, regulatory compliance, and detecting potential adulteration or off-spec fermentation. This test method identifies isopropanol, N-propanol, isobutanol, isoamyl alcohol, and N-amyl alcohol.

Enterobacteriaceae

This test method is a standardized microbiological procedure for the detection and enumeration of Enterobacteriaceae in food and environmental samples. This method utilizes selective culture media and incubation conditions to isolate and quantify members of this important bacterial family.

D-Mannose

This test method is an enzymatic spectrophotometric method that quantifies D‑mannose by converting sugars through sequential enzyme reactions. It provides a specific, sensitive determination of mannose in plant products and polysaccharide hydrolysates.

Clostridium

This test method is a presumptive quantification of clostridium spp. in fermentation samples found within the ethanol production process.