This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino… Continue reading Amino Acids [Main] Pre-col AQC Der
This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino… Continue reading Amino Acids [Taurine] Pre-col AQC Der
This test method involves chemically modifying tryptophan to form stable, UV- or fluorescence-detectable derivatives before chromatographic separation. Because tryptophan is sensitive to acid hydrolysis, alkaline hydrolysis is commonly used to release tryptophan without degradation. The derivatized tryptophan is then separated on a reversed-phase UPLC column and detected using UV or fluorescence detectors. This method allows… Continue reading Amino Acids [Tryptophan] Pre-col AQC Der
This test method is a widely used technique to accurately quantify these sulfur-containing amino acids in food, feed, and biological samples. Because cysteine and methionine are sensitive to oxidation and do not naturally absorb UV light strongly, they require chemical modification (derivatization) prior to chromatographic analysis. Samples are first oxidized to stabilize these amino acids,… Continue reading Amino Acids [Cysteine/Methionine] Pre-col AQC Der