This test method quantitatively analyzes vitamin B-7 (biotin) in complex food and supplement matrices using UPLC-MS technology. By combining UPLC separation with mass detection, this method achieves superior sensitivity and selectivity compared to UV-only approaches, enabling reliable biotin quantification even at trace levels.
This test method quantitatively analyzes vitamin B-9 (folic acid) in complex food and supplement matrices using UPLC-MS technology. By combining UPLC separation with mass detection, this method achieves superior sensitivity and selectivity compared to UV-only approaches, enabling reliable biotin quantification even at trace levels.
This USP test method for Ascorbic Acid Assay is a standardized method to quantitatively determine the amount of ascorbic acid (vitamin C) in a sample.
This test method determines the color of oils and other transparent liquids by comparing samples to calibrated Lovibond® or Gardner liquid standards, providing a standardized visual evaluation of red/yellow (RYBN) tint and overall Gardner color to ensure consistent color grading across batches.
This test method is an official gravimetric procedure for determining calcium carbonate (CaCO₃) content in samples such as minerals, supplements, and food additives. The method involves acid digestion of the sample and measurement of the resulting carbon dioxide to quantify the effective calcium carbonate equivalence.
This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino… Continue reading Amino Acids [Taurine] Pre-col AQC Der
This test method involves chemically modifying tryptophan to form stable, UV- or fluorescence-detectable derivatives before chromatographic separation. Because tryptophan is sensitive to acid hydrolysis, alkaline hydrolysis is commonly used to release tryptophan without degradation. The derivatized tryptophan is then separated on a reversed-phase UPLC column and detected using UV or fluorescence detectors. This method allows… Continue reading Amino Acids [Tryptophan] Pre-col AQC Der
This test method involves chemically modifying amino acids after hydrolysis to form stable, UV- or fluorescence-detectable derivatives. This enhances detection sensitivity and allows efficient separation using reversed-phase UPLC columns. Samples undergo acid hydrolysis to release free amino acids, which are then reacted with a derivatizing reagent (e.g., AccQ•Tag™) to form stable derivatives. These derivatized amino… Continue reading Amino Acids [Main] Pre-col AQC Der
This test method is a widely used technique to accurately quantify these sulfur-containing amino acids in food, feed, and biological samples. Because cysteine and methionine are sensitive to oxidation and do not naturally absorb UV light strongly, they require chemical modification (derivatization) prior to chromatographic analysis. Samples are first oxidized to stabilize these amino acids,… Continue reading Amino Acids [Cysteine/Methionine] Pre-col AQC Der