This test method is a cross-method calculation used to determine the energy content of a sample, utilizing total carbohydrate, crude fat, and crude protein analyses in the “4-9-4 rule,” where there are 4 calories per gram of carbohydrates, 9 calories per gram of fat, and 4 calories per gram of protein in a sample.
This test method is a cross-method calculation used to determine the total carbohydrate content of a sample, utilizing crude protein, crude fat, moisture, and ash analyses to calculate total carbohydrates by difference. The calculation assumes that the remainder after accounting for the protein, fat, moisture, and ash represents carbohydrates, including sugars, starches, and dietary fiber.
This test method is a validated gas chromatography (GC) method for determining cholesterol content in a wide range of foods, including meats, dairy products, and processed items. It involves extraction, saponification, and derivatization steps to isolate cholesterol from the sample matrix, followed by quantification using a GC with a flame ionization detector (FID).
This test method is a presumptive quantification of clostridium spp. in fermentation samples found within the ethanol production process.
This test method is a gravimetric method used to determine the crude ash content of animal feed. Ash represents the total mineral content in a sample and is determined by incinerating the sample at high temperature to burn off all organic matter. The remaining inorganic residue (ash) is weighed to calculate the percentage of ash… Continue reading Ash
This test method is a simple, packed, volumetric technique used to measure the mass per unit volume of a granular or powdered material, including any voids between particles. This value is important for evaluating flow properties, packaging efficiency, and handling characteristics of materials like grains, powders, or animal feed.
This test method is a classic analytical technique used to determine ammonia nitrogen (NH₃-N) in feed samples. It involves alkalizing the sample to convert ammonium ions to ammonia gas, which is then distilled, captured in an acid solution, and quantified by titration. This method measures the concentration of ammonia as nitrogen and is widely used… Continue reading Ammonium Nitrogen [Feed]
This test method is used to determine the content of ammonia nitrogen and urea in animal feed, both of which are non-protein nitrogen (NPN) sources added to ruminant feed to support microbial protein synthesis.
This test method is a procedure used to determine Acid Detergent Lignin (ADL) in feedstuffs, forages, and fibrous materials. This method measures the lignin content—a complex, indigestible component of plant cell walls—after removing other fibrous materials. Lignin is important because it negatively impacts digestibility and nutrient availability in ruminant diets. The sample is treated with… Continue reading Acid Detergent Lignin [ADL]
This test method is a modified version of the standard ether extraction method for determining crude fat in animal feed and feed ingredients. This modification is used when samples contain bound fats (e.g., in high-fiber or heat-processed feeds) that are not fully extractable by ether alone. The sample is first treated with hydrochloric acid (HCl)… Continue reading Acid Hydrolysis Fat